Evasion of silencing by a hairpin can be achieved through mutagenesis within the target area22, 26, 33 and such an engineered cDNA could be used for future applications of suppression and replacement, which is the process of gene supplementation with an engineered cDNA that is not silenced by the target hairpin after all endogenous expression has been abolished. Hence, the first three triplets within the first eight nucleotides or region of RDS6 were altered to abolish shRNA:mRNA binding while retaining the wild type amino acid sequence (Figure 2). The miRNA template used was miR 30a34 and the hairpin was named miRDS6.
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Forty four cups hold only sugar cubes representing those under age 18, too young for the ritual, who had died. The installation is enhanced by recordings of the artist reciting the names and birthdates of the victims.The making and serving of coffee will take place from 12 pm to 5 pm beginning today, 11 July, over a period of five days, representing the number of days the massacre lasted, and will remain on display until 29 July. Other elements forming part of the exhibit are a collection of about 40 photographs by Bosnian photographers, a slide show showing the photographs in the exhibition, as well as a five minute film showing the same installation being conducted by the artist in Sarajevo last year.The exhibition is sponsored by the Permanent Mission of Bosnia and Herzegovina to the United Nations, organized by the Academy of Bosnia and Herzegovina, Inc., and supported by a grant from the Open Society Institute..
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